Caution urged for the use of gene-editing technology CRISPR – HDBuzz


By Dr Rachel Harding Edited by Dr Leora Fox

A gene-editing tool known as CRISPR has been heralded as a breakthrough technology for scientists in the lab but also as a potential strategy to treat numerous genetic diseases, including Huntington’s. But a series of recent studies has suggested that CRISPR is less precise than previously thought, leading to unintended changes in the genome. Three independent studies which each sought to edit a single gene have shown that other parts of the DNA were also unexpectedly changed.

These studies are presented in an unfinalized online format and are still working their way through the scientific peer review process before formal publication. This means that there may still be errors in the science presented in the manuscripts which have yet to be spotted. However, as these independent and parallel studies all corroborate the finding that CRISPR technology can have off-target effects, there has already been mainstream and scientific media attention to these results. Although CRISPR still holds much promise as a research tool, it seems it is still a way off from being ready to be used as a medicine.

CRISPR technology allows scientists to make edits to our genomes so many researchers are excited to try and use this technology to treat genetic diseases like Huntington’s disease

Image credit: Quinn Dombrowski

The CRISPR system was first discovered as a type of immune system used by bacteria to defend themselves against viruses and other invaders by chopping up foreign genetic material. There are two components to the CRISPR machinery: A special piece of genetic message called a guide RNA that finds and points to the invader’s DNA, and an enzyme that can cut the DNA to destroy it. Check out this previous HDBuzz article for a detailed look at how CRISPR works and the challenges faced by the researchers exploring it.

Scientists can manipulate the system by changing the guide RNA to a target sequence and can therefore use the CRISPR system to make edits at precise parts of the genome. These edits are permanent changes to the genome and will be passed down from one generation to the next. Since its discovery in the late 2000s, scientists have been dreaming up all sorts of ideas and uses for CRISPR. For example, could it be possible to edit out the HD gene expansion in embryos? These ideas are not without controversy given the ethical dilemmas surrounding genetic manipulation of humans or other species. CRISPR hit the headlines in 2018 when the CRISPR experiments of rogue scientist He Jiankui at the Southern University of Science and Technology in China resulted in the birth of twin girls who had their genomes edited.

CRISPR gene editing could lead to very big and unintended changes to other parts of the genome

The three recent studies were conducted by scientists at the Francis Crick Institute in London, Columbia University in New York City and Oregon Health & Science University in Portland. Each team of scientists used CRISPR to make different edits in human embryos donated to research. The three experiments targeted genes involved in fetus development, blindness and heart problems. All of the studies found that CRISPR gene editing could lead to very big and unintended changes to other parts of the genome, including large deletions and rearrangements in regions close to the target gene. This is bad because our DNA code is a very precise set of instructions, which can be thought of like a cooking recipe. If you rearranged the steps in a recipe or got rid of some of the ingredients the outcome would not be good!

These off-target effects are due to problems in repairing the DNA, although there is not consensus amongst the studies as to how that might be happening. One thing all of the scientists do agree on though is that this should give everyone pause for thought when considering using CRISPR for genome editing as the technology is not as fine-tuned as previously thought.

The edits made to DNA by CRISPR are permanent and can be passed down from one generation to the next
The edits made to DNA by CRISPR are permanent and can be passed down from one generation to the next

Image credit: ZEISS Microscopy

The permanent nature of CRISPR-based therapies for genetic illnesses like HD is both attractive and daunting. In theory, CRISPR could be used to irreversibly cut out some of the CAG repeats in the expanded huntingtin gene within an embryo. That way, the person who grows from that embryo wouldn’t have HD and neither would their children. The permanent nature of edits made by CRISPR technology means that unintended consequences would not only affect the individual, but would also be passed from one generation to the next. Scientists are not keen to roll out the technology for these type of gene-therapies until they are absolutely certain that it is safe and working as planned.

The good news is that these recent discoveries have alerted scientists to the limitations of CRISPR technology. This will by no means stop scientists from dreaming big about how CRISPR could be useful in the future, but the community will move forward more cautiously. Scientists will now consider how best to improve the technology to avoid these types of problems and hopefully use CRISPR as a safe therapy for different genetic illnesses in the future.



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